haploid induction

Three new haploid inducers were developed for screening immature haploid embryos during in vivo haploid induction. MAS of both qhir1 and qhir8 was used for inducers breeding, the efficiency improve greatly.

Here, we present the discovery that mutation of a non-Stock6-originating gene in qhir8, namely, ZmDMP, enhances and triggers haploid induction. ZmDMP was identified by map-based cloning and further verified by CRISPR–Cas9-mediated knockout experiments.